Journal: Nutrients

Article Title: Bioactive Oligopeptides from Ginseng ( Panax ginseng Meyer) Suppress Oxidative Stress-Induced Senescence in Fibroblasts via NAD + /SIRT1/PGC-1α Signaling Pathway

doi: 10.3390/nu14245289

Figure Lengend Snippet: Effect of GOPs on the senescence-associated secretory phenotype (SASP). ( A ) Effect of GOPs on the interleukin-6 (IL-6) concentration. ( B ) Effect of GOPs on the IL-1β concentration. ( C ) Effect of GOPs on the matrix metalloproteinase-3 (MMP-3) concentration. ( D ) Effect of GOPs on the intercellular cell adhesion molecule-1 (ICAM-1) concentration. ( E ) Effect of GOPs on the vascular cell adhesion molecule-1 (VCAM-1) concentration. ( F ) Effect of GOPs on the activation of p-NF-κB/NF-κB. Values represent the mean ± S.D. ( n = 3 per group). # p < 0.05 versus control group, * p < 0.05 versus model group.

Article Snippet: Then, the supernatant was obtained for the measurements of malondialdehyde (MDA) (Nanjing Jiancheng, Nanjing, China), glutathione peroxidase (GSH-Px) (Nanjing Jiancheng, Nanjing, China), superoxide dismutase (SOD) (Nanjing Jiancheng, Nanjing, China) activities, NAD + /NADH (Beyotime, Shanghai, China), telomerase (TE) activity (FEIYA, Jiangsu, China), interleukin-6 (IL-6) (Invitrogen, Waltham, MA, USA), IL-1β (Invitrogen, Waltham, MA, USA), matrix metalloproteinase-3 (MMP-3) (Multisciences, Hangzhou, China), intercellular cell adhesion molecule-1 (ICAM-1) (Multisciences, Hangzhou, China), and vascular cell adhesion molecule-1 (VCAM-1) (Multisciences, Hangzhou, China) using commercial kits.

Techniques: Concentration Assay, Activation Assay